Week 2 >> Assessment
When comparing genetic datasets of different related algal species, highly conserved sequences may represent functionally important gene sequences.
Electropolation is usually used for modifying the chloroplast genome.
Bolistic delivery via gene guns are usually used for modifying the chloroplast genome.
What is synthetic biology?
What is bio-prospecting?
What is gene shuffling?
A flow cytometer and a cell sorter are used for single cell isolation during breeding selection.
Cyanobacteria are prokaryotic, meaning they do not have a nucleus.
Scientific evidence suggests that cyanobacteria are the evolutionary precursors to algal and terrestrial plant chloroplasts.
Cyanobacteria can undergo gene conjugation, meaning they get genes from the environment.
They can undergo conjugation, but this is the process of gaining genes from other microbes.
Why are some genetic tools for cyanobacteria not universal?
Algae chloroplasts have a complete genetic system.
Gene expression in chloroplast is primarily controlled during transcription.
It is controlled during translation.
The type of promoter used during genetic engineering of chloroplast has a large effect on how much recombinant protein is expressed.
The 5 prime untranslated region has a large impact.
Complex systems, such as photosynthesis, can be synthetically engineered in the chloroplast.
All amino acids are encoded by a single unique combination of three nucleotides called “codon”.
The same codon will always encode for the same amino acid, but each amino acid can be encoded by different codons as is represented in the codon table.
The majority of genes in green algae are found in their nuclei.
Over 99% of their genes are in the nucleus.